College of Education for Pure Science - EDUPS

Master Thesis Viva in the College of Education for Pure Science entitled "Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice "

The College of Education for Pure Science, University of Mosul, has done the Master Thesis Viva entitled "Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice",

On Thursday, February 2, 2023, the respected Dean of the College, Assistant Professor Dr. Qais Ismail Ibrahim, the Honorable Scientific Associate and Administrative Associate, the Honorable Head of the Department of Biology, and a number of the college's teachers were attended the viva.

     The current study, presented by the M.Sc. Student Sarah Abdulwahd Mohammed Al- badrani in the Department of Biology, investigated the effect of Selenium nanoparticles on the viability of Protoscoleces of Echinococcus granulosus in vitro by applying different concentrations 50- 100- 150- 200- 250- 350-500 µg/ ml for different exposure time 10- 20- 30- 60 minutes, Four concentrations were selected and injected into mice (in vivo) , on the immune response of BALB/c mice against infection with cystic echinococcosis in contrast to the control group, injected with un exposure Protoscoleces to selenium nanoparticles , depending on many criteria including changes in the numbers, weights and diameters of hydatid cysts, changes in the weights of liver and spleen and their organ index, measurement of immune cytokines in the serum of the experimental animals. The study period lasted for five months, in which 75 male mice were used.

The results revealed :

1. An increase in the mortality rate of Protoscoleces in vitro , in proportional with exposure duration of selenium nanoparticles.

2. A reduction of the hydatid cysts numbers with ratios of %78.1,% 85.3, 88.6% 90.6% in the groups of mice for all concentrations that vitality rate of the protoscoleces exposed to selenium nanoparticles was 98% after five months of infection.

3. A Reduction significant (P≤ 0.001) in the hydatid cysts numbers, weights and diameters of treated mice, the minimum number was 0.8after five months of infection , the lowest diameters was 0.2880 mm whereas in the control was 2.1760 mm , whereas the minimum weight was 0.0320 mg compared to the control 0.300 mg after five months of infection.

4. A significant reduction (P≤ 0.001) in the liver and spleen weights, in treated mice, the minimum weight of the liver was the 1.1140 gm compared to the control 1.5840gm with the organ index of 22.874 gm compared to the control 48.129 gm . concerning the weights of the spleen there were no significant differences between treated and control groups, while the organ index had a significant differences (P ≤ 0.005) with the minimum organ index in the treated group 3.0126 in comparison to the control 5.1396 five months post infection .

5. A significant increase (P≤ 0.001) in the immune response of treated mice, represented by a significant decrease in the levels of IL -6 (18.461) pg/ml and IL -12 (14.565) ng/L in comparison with the control group (66.548) pg/ml , (146.125) ng/L, respectively, an Increase in TNF- α level in treat mice (147.693) ng/L compared to the control group (92.593) ng/L , five months post infection.

The Viva committee was chaired by Prof. Dr. Adel Taleb Mohammed / University of Duhok /College of Medicine and the membership of Prof. Dr. Sundus Natheer Hameed/ University of Mosul / College of Science, Asst. Prof. Dr. Ibrahim Faris Ali / University of Mosul / College of Education for Pure Science and under the supervision and membership of Prof. Dr. Asmaa Abdulaziz Ali / University of Mosul / College of Education for Pure Science.